Journal: RNA

Article Title: Nano LC-MS using capillary columns enables accurate quantification of modified ribonucleosides at low femtomol levels

doi: 10.1261/rna.065482.117

Figure Lengend Snippet: Reliable ribonucleoside separation is achieved by reversed-phase chromatography using porous graphitic carbon (PGC) and C18 materials. ( A ) Representative chromatograms (A 254 -trace) obtained with PGC-A ( left panel), PGC-B ( middle panel), and C18 ( right panel) separating 20 ribonucleoside standards (LSM, Supplemental Table 1 ). ( B ) Individual chromatograms obtained for each of the 20 ribonucleosides included in the LSM using run conditions as in A . Abbreviations follow the Modomics database convention .

Article Snippet: The PGC-A (2.1 × 150 mm), PGC-B (2.1 × 100 mm), and C18 (2.0 × 150 mm) columns were connected to a Knauer PLATINblue UPLC system equipped with an Autosampler 3950, a PLATINBlue T-1 column thermostat, and a MW-1 UV detector (KNAUER Wissenschaftliche Geräte GmbH).

Techniques: Reversed-phase Chromatography

Journal: RNA

Article Title: Nano LC-MS using capillary columns enables accurate quantification of modified ribonucleosides at low femtomol levels

doi: 10.1261/rna.065482.117

Figure Lengend Snippet: Ribonucleosides separation is robust and reproducible on PGC materials. Retention time and column durability analysis of 30 consecutive runs with pseudouridine (Ψ), 5-methylcytidine (m 5 C), inosine (I), adenosine (A), and 1-methylguanosine (m 1 G) on PGC-A and PGC-B columns. Representative chromatograms from runs 1, 5, 15, and 30 are shown. The table summarizes the retention time parameters for all tested nucleosides. Abbreviations follow the Modomics database convention .

Article Snippet: The PGC-A (2.1 × 150 mm), PGC-B (2.1 × 100 mm), and C18 (2.0 × 150 mm) columns were connected to a Knauer PLATINblue UPLC system equipped with an Autosampler 3950, a PLATINBlue T-1 column thermostat, and a MW-1 UV detector (KNAUER Wissenschaftliche Geräte GmbH).

Techniques:

Journal: RNA

Article Title: Nano LC-MS using capillary columns enables accurate quantification of modified ribonucleosides at low femtomol levels

doi: 10.1261/rna.065482.117

Figure Lengend Snippet: PGC and C18 capillaries enable the analysis of ribonucleosides in nLC ESI-MS/MS. ( A ) Representative total ion chromatograms (TIC) of 100 ng of the LSM or CSM (C18 only) analyzed on nanoflow capillary columns packed with PGC-A ( left panel), PGC-B ( middle panel), or C18 ( right panel) material. ( B ) Extracted ion chromatograms (XIC) for adenosine (A), cytidine (C), guanosine (G), uridine (U), pseudouridine (Ψ), inosine (I), N 4 -acetylcytidine (ac 4 C), 1-methyladenosine (m 1 A), 2′- O -methyladenosine (Am), and N 6 -methyladenosine (m 6 A; CSM only). ( C ) Example MS1 spectra recorded at the retention times corresponding to the nucleosides analyzed in B . (NL) Normalized target level.

Article Snippet: The PGC-A (2.1 × 150 mm), PGC-B (2.1 × 100 mm), and C18 (2.0 × 150 mm) columns were connected to a Knauer PLATINblue UPLC system equipped with an Autosampler 3950, a PLATINBlue T-1 column thermostat, and a MW-1 UV detector (KNAUER Wissenschaftliche Geräte GmbH).

Techniques: Tandem Mass Spectroscopy

Journal: iScience

Article Title: Atrial natriuretic peptide signaling co-regulates lipid metabolism and ventricular conduction system gene expression in the embryonic heart

doi: 10.1016/j.isci.2023.108748

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal PGCA antibody , FabGennix , Cat#PGCA-101AP; RRID: AB_2315119.

Techniques: Diagnostic Assay, Recombinant, Modification, Membrane, Software